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Merck & Co silica gel column chromatography
Silica Gel Column Chromatography, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/silica+gel+column+chromatography/gel+silica+%EC%9D%98/10__1016_slash_j__bse__2026__105275-64-18-22
Average 86 stars, based on 1 article reviews
silica gel column chromatography - by Bioz Stars, 2026-09
86/100 stars

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Purification:

Article Title: Design, Synthesis, and Pharmacokinetic Profiling of Fluorinated Reversible N -Alkyl Carbamate Derivatives of Psilocin for Sub-Hallucinogenic Brain Exposure.
Article Snippet: .. Compound purification was performed by silica gel column chromatography (220−440 mesh, 60 Å pore size, Merck, positive nitrogen pressure), or by reverse phase semipreparative HPLC (Agilent 1260 Infinity II, column Phenomenex Luna C18, 5 μm, 100 Å, 250 × 21.2 mm, elution with water +0.1% TFA/ACN). .. 1H and 13C nuclear magnetic resonance (NMR) spectra were acquired on a Bruker Avance III HD 400 spectrometer operating at 400 MHz for 1H and 101 MHz for 13C.

Article Title: Antimicrobial and Anticancer Properties of Phenazines from Streptomyces murinus ZMA01, an Endophyte in Zea mays L.
Article Snippet: Chloramphenicol (bacterial positive control) and cycloheximide (fungal positive control), both sourced from Thermo Fisher Scientific, were included to validate the assay. .. Compound purification and characterization: Fractionation of the crude extract (20.00 g) was achieved through silica gel column chromatography (Merck, 0.040-0.063 mm). ..

Article Title: Design, Synthesis, and Pharmacokinetic Profiling of Fluorinated Reversible N ‑Alkyl Carbamate Derivatives of Psilocin for Sub-Hallucinogenic Brain Exposure
Article Snippet: .. Compound purification was performed by silica gel column chromatography (220–440 mesh, 60 Å pore size, Merck, positive nitrogen pressure), or by reverse phase semipreparative HPLC (Agilent 1260 Infinity II, column Phenomenex Luna C18, 5 μm, 100 Å, 250 × 21.2 mm, elution with water +0.1% TFA/ACN). .. 1 H and 13 C nuclear magnetic resonance (NMR) spectra were acquired on a Bruker Avance III HD 400 spectrometer operating at 400 MHz for 1 H and 101 MHz for 13 C. 19 F NMR spectra were acquired on a Bruker Avance III HD 400 spectrometer operating at 376 MHz or a Bruker 200 spectrometer operating at 188 MHz.

Article Title: Antibacterial and Anticancer Properties of Anthraquinone Derivatives from Streptomyces kunmingensis BH111, an Isolate from Beehive.
Article Snippet: .. Compound purification and characterization: For purification, 9.21 g of the crude extract was subjected to silica gel column chromatography (Merck, 0.040-0.063 mm). ..

Article Title: Discovery of Potent Antibacterial and Anticancer Flavonoids from Beehive-Associated Streptomyces griseoaurantiacus HNF214.
Article Snippet: .. Compound purification and characterization: For the purification of bioactive compounds, 18 g of the crude extract was subjected to silica gel column chromatography (Merck, 0.040-0.063 mm). ..

Column Chromatography:

Article Title: Design, Synthesis, and Pharmacokinetic Profiling of Fluorinated Reversible N -Alkyl Carbamate Derivatives of Psilocin for Sub-Hallucinogenic Brain Exposure.
Article Snippet: .. Compound purification was performed by silica gel column chromatography (220−440 mesh, 60 Å pore size, Merck, positive nitrogen pressure), or by reverse phase semipreparative HPLC (Agilent 1260 Infinity II, column Phenomenex Luna C18, 5 μm, 100 Å, 250 × 21.2 mm, elution with water +0.1% TFA/ACN). .. 1H and 13C nuclear magnetic resonance (NMR) spectra were acquired on a Bruker Avance III HD 400 spectrometer operating at 400 MHz for 1H and 101 MHz for 13C.

Article Title: Antimicrobial and Anticancer Properties of Phenazines from Streptomyces murinus ZMA01, an Endophyte in Zea mays L.
Article Snippet: Chloramphenicol (bacterial positive control) and cycloheximide (fungal positive control), both sourced from Thermo Fisher Scientific, were included to validate the assay. .. Compound purification and characterization: Fractionation of the crude extract (20.00 g) was achieved through silica gel column chromatography (Merck, 0.040-0.063 mm). ..

Article Title: Design, Synthesis, and Pharmacokinetic Profiling of Fluorinated Reversible N ‑Alkyl Carbamate Derivatives of Psilocin for Sub-Hallucinogenic Brain Exposure
Article Snippet: .. Compound purification was performed by silica gel column chromatography (220–440 mesh, 60 Å pore size, Merck, positive nitrogen pressure), or by reverse phase semipreparative HPLC (Agilent 1260 Infinity II, column Phenomenex Luna C18, 5 μm, 100 Å, 250 × 21.2 mm, elution with water +0.1% TFA/ACN). .. 1 H and 13 C nuclear magnetic resonance (NMR) spectra were acquired on a Bruker Avance III HD 400 spectrometer operating at 400 MHz for 1 H and 101 MHz for 13 C. 19 F NMR spectra were acquired on a Bruker Avance III HD 400 spectrometer operating at 376 MHz or a Bruker 200 spectrometer operating at 188 MHz.

Article Title: Antibacterial and Anticancer Properties of Anthraquinone Derivatives from Streptomyces kunmingensis BH111, an Isolate from Beehive.
Article Snippet: .. Compound purification and characterization: For purification, 9.21 g of the crude extract was subjected to silica gel column chromatography (Merck, 0.040-0.063 mm). ..

Article Title: A novel ellagic acid trimer and phenolic constituents from the trunk bark of Rinorea oblongifolia (Violaceae) and their chemotaxonomic significance
Article Snippet: The phytochemical investigation of the ethyl acetate fraction derived from the trunk bark of Rinorea oblongifolia (Violaceae) led to the isolation of ten phenolic compounds, including a new O,O′-linked ellagic acid trimer (1), together with nine known compounds: 3′,3,4-tri-O-methylellagic acid 4′-O-β-D-glucopyranoside (2), 3,3′-di-Omethylellagic acid (3), 3′,4,5,5′-tetramethylcoruleoellagic acid (4), gallic acid (5), methyl gallate (6), 4-hydroxy3,5-dimethoxybenzoic acid (7), kaempferol (8), kaempferol 3-O-rutinoside (9), and (− )-epicatechin (10).. Their structures were elucidated using comprehensive spectroscopic analyses, including nuclear magnetic resonance (NMR) and mass spectrometry.. The predominance of ellagic acid derivatives in this fraction is consistent with previous studies on non-polar extracts of this species and highlights the importance of ellagic acid-based phenolics as potential chemotaxonomic markers for the genus Rinorea and the family Violaceae.

Article Title: Comparative Anticancer Activity of Extract, Partitions, and a Two-Acetogenin Mixture from Mexican Creole Avocado Seed
Article Snippet: .. AM (avocadene acetate and avocadyne acetate) was isolated from 5.2 g of HP, separated by silica gel column chromatography (15 × 5.5 cm, Merck, Boston, MA, USA), eluted sequentially with 250 mL of hexane, followed by 250 mL each of the mixtures hexane/ethyl acetate (9:1), (8:2), (65:35), (1:1), and (3:7). .. Thirty fractions of 55 mL each were collected and monitored by thin-layer chromatography (TLC; Merck 60 F254, Boston, MA, USA), fractions showing similar chromatographic profiles were combined and rotary evaporated (Büchi R-114, Flawil, Switzerland).

Article Title: Comparative Anticancer Activity of Extract, Partitions, and a Two-Acetogenin Mixture from Mexican Creole Avocado Seed.
Article Snippet: .. AM (avocadene acetate and avocadyne acetate) was isolated from 5.2 g of HP, separated by silica gel column chromatography (15 × 5.5 cm, Merck, Boston, MA, USA), eluted sequentially with 250 mL of hexane, followed by 250 mL each of the mixtures hexane/ethyl acetate (9:1), (8:2), (65:35), (1:1), and (3:7). .. Thirty fractions of 55 mL each were collected and monitored by thin-layer chromatography (TLC; Merck 60 F254, Boston, MA, USA), fractions showing similar chromatographic profiles were combined and rotary evaporated (Büchi R114, Flawil, Switzerland).

Article Title: Discovery of Potent Antibacterial and Anticancer Flavonoids from Beehive-Associated Streptomyces griseoaurantiacus HNF214.
Article Snippet: .. Compound purification and characterization: For the purification of bioactive compounds, 18 g of the crude extract was subjected to silica gel column chromatography (Merck, 0.040-0.063 mm). ..

Pore Size:

Article Title: Design, Synthesis, and Pharmacokinetic Profiling of Fluorinated Reversible N -Alkyl Carbamate Derivatives of Psilocin for Sub-Hallucinogenic Brain Exposure.
Article Snippet: .. Compound purification was performed by silica gel column chromatography (220−440 mesh, 60 Å pore size, Merck, positive nitrogen pressure), or by reverse phase semipreparative HPLC (Agilent 1260 Infinity II, column Phenomenex Luna C18, 5 μm, 100 Å, 250 × 21.2 mm, elution with water +0.1% TFA/ACN). .. 1H and 13C nuclear magnetic resonance (NMR) spectra were acquired on a Bruker Avance III HD 400 spectrometer operating at 400 MHz for 1H and 101 MHz for 13C.

Article Title: Design, Synthesis, and Pharmacokinetic Profiling of Fluorinated Reversible N ‑Alkyl Carbamate Derivatives of Psilocin for Sub-Hallucinogenic Brain Exposure
Article Snippet: .. Compound purification was performed by silica gel column chromatography (220–440 mesh, 60 Å pore size, Merck, positive nitrogen pressure), or by reverse phase semipreparative HPLC (Agilent 1260 Infinity II, column Phenomenex Luna C18, 5 μm, 100 Å, 250 × 21.2 mm, elution with water +0.1% TFA/ACN). .. 1 H and 13 C nuclear magnetic resonance (NMR) spectra were acquired on a Bruker Avance III HD 400 spectrometer operating at 400 MHz for 1 H and 101 MHz for 13 C. 19 F NMR spectra were acquired on a Bruker Avance III HD 400 spectrometer operating at 376 MHz or a Bruker 200 spectrometer operating at 188 MHz.

High Performance Liquid Chromatography:

Article Title: Design, Synthesis, and Pharmacokinetic Profiling of Fluorinated Reversible N -Alkyl Carbamate Derivatives of Psilocin for Sub-Hallucinogenic Brain Exposure.
Article Snippet: .. Compound purification was performed by silica gel column chromatography (220−440 mesh, 60 Å pore size, Merck, positive nitrogen pressure), or by reverse phase semipreparative HPLC (Agilent 1260 Infinity II, column Phenomenex Luna C18, 5 μm, 100 Å, 250 × 21.2 mm, elution with water +0.1% TFA/ACN). .. 1H and 13C nuclear magnetic resonance (NMR) spectra were acquired on a Bruker Avance III HD 400 spectrometer operating at 400 MHz for 1H and 101 MHz for 13C.

Article Title: Design, Synthesis, and Pharmacokinetic Profiling of Fluorinated Reversible N ‑Alkyl Carbamate Derivatives of Psilocin for Sub-Hallucinogenic Brain Exposure
Article Snippet: .. Compound purification was performed by silica gel column chromatography (220–440 mesh, 60 Å pore size, Merck, positive nitrogen pressure), or by reverse phase semipreparative HPLC (Agilent 1260 Infinity II, column Phenomenex Luna C18, 5 μm, 100 Å, 250 × 21.2 mm, elution with water +0.1% TFA/ACN). .. 1 H and 13 C nuclear magnetic resonance (NMR) spectra were acquired on a Bruker Avance III HD 400 spectrometer operating at 400 MHz for 1 H and 101 MHz for 13 C. 19 F NMR spectra were acquired on a Bruker Avance III HD 400 spectrometer operating at 376 MHz or a Bruker 200 spectrometer operating at 188 MHz.

Fractionation:

Article Title: Antimicrobial and Anticancer Properties of Phenazines from Streptomyces murinus ZMA01, an Endophyte in Zea mays L.
Article Snippet: Chloramphenicol (bacterial positive control) and cycloheximide (fungal positive control), both sourced from Thermo Fisher Scientific, were included to validate the assay. .. Compound purification and characterization: Fractionation of the crude extract (20.00 g) was achieved through silica gel column chromatography (Merck, 0.040-0.063 mm). ..

Isolation:

Article Title: A novel ellagic acid trimer and phenolic constituents from the trunk bark of Rinorea oblongifolia (Violaceae) and their chemotaxonomic significance
Article Snippet: The phytochemical investigation of the ethyl acetate fraction derived from the trunk bark of Rinorea oblongifolia (Violaceae) led to the isolation of ten phenolic compounds, including a new O,O′-linked ellagic acid trimer (1), together with nine known compounds: 3′,3,4-tri-O-methylellagic acid 4′-O-β-D-glucopyranoside (2), 3,3′-di-Omethylellagic acid (3), 3′,4,5,5′-tetramethylcoruleoellagic acid (4), gallic acid (5), methyl gallate (6), 4-hydroxy3,5-dimethoxybenzoic acid (7), kaempferol (8), kaempferol 3-O-rutinoside (9), and (− )-epicatechin (10).. Their structures were elucidated using comprehensive spectroscopic analyses, including nuclear magnetic resonance (NMR) and mass spectrometry.. The predominance of ellagic acid derivatives in this fraction is consistent with previous studies on non-polar extracts of this species and highlights the importance of ellagic acid-based phenolics as potential chemotaxonomic markers for the genus Rinorea and the family Violaceae.

Article Title: Comparative Anticancer Activity of Extract, Partitions, and a Two-Acetogenin Mixture from Mexican Creole Avocado Seed
Article Snippet: .. AM (avocadene acetate and avocadyne acetate) was isolated from 5.2 g of HP, separated by silica gel column chromatography (15 × 5.5 cm, Merck, Boston, MA, USA), eluted sequentially with 250 mL of hexane, followed by 250 mL each of the mixtures hexane/ethyl acetate (9:1), (8:2), (65:35), (1:1), and (3:7). .. Thirty fractions of 55 mL each were collected and monitored by thin-layer chromatography (TLC; Merck 60 F254, Boston, MA, USA), fractions showing similar chromatographic profiles were combined and rotary evaporated (Büchi R-114, Flawil, Switzerland).

Article Title: Comparative Anticancer Activity of Extract, Partitions, and a Two-Acetogenin Mixture from Mexican Creole Avocado Seed.
Article Snippet: .. AM (avocadene acetate and avocadyne acetate) was isolated from 5.2 g of HP, separated by silica gel column chromatography (15 × 5.5 cm, Merck, Boston, MA, USA), eluted sequentially with 250 mL of hexane, followed by 250 mL each of the mixtures hexane/ethyl acetate (9:1), (8:2), (65:35), (1:1), and (3:7). .. Thirty fractions of 55 mL each were collected and monitored by thin-layer chromatography (TLC; Merck 60 F254, Boston, MA, USA), fractions showing similar chromatographic profiles were combined and rotary evaporated (Büchi R114, Flawil, Switzerland).



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